5/26/2023 0 Comments Four Fold Serial Dilutions![]() Taken together, our results show that porins are strong immunogens that confer lifelong specific bactericidal antibody responses in the absence of added adjuvant. These results indicate that OmpC is the main protein responsible for the antibody-mediated memory bactericidal response induced by porins. ![]() In contrast, although OmpC induced sustained high bactericidal antibody titres for 300 days, postimmunization, the bactericidal antibody titre induced by OmpF was not detected at day 180. OmpC and OmpF induced long-lasting antibody titres, measured by ELISA, which were sustained for 300 days. Because our porin preparations contained the outer membrane proteins C and F (OmpC and OmpF), we evaluated the individual contribution of each porin to the long-lasting antibody response. typhi strain, whereas porin-specific antibody titres measured by enzyme-linked immunosorbent assay (ELISA) decreased with time. Notably, the antiporin sera showed a sustained lifelong bactericidal-binding activity to the wild-type S. typhi porin sera did not cross-react with S. Despite the high homology of porins, the long-lasting anti- S. Analysis of the IgG subclasses produced during this antibody response revealed the presence of the subclasses IgG2b, IgG1, IgG2a and weak IgG3. These porins triggered a strong lifelong production of immunoglobulin G (IgG) antibody in the absence of exogenous adjuvant. We examined the ability of porins from Salmonella enterica serovar typhi to induce a long-term antibody response in BALB/c mice.
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